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accessarray barcoded primers  (fluidigm)


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    Structured Review

    fluidigm accessarray barcoded primers
    Accessarray Barcoded Primers, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessarray+primers/accessarray+barcoding+system+primers/pmc11362537-258-7-4
    Average 90 stars, based on 1 article reviews
    accessarray barcoded primers - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Assessing the comparability of different DNA extraction and amplification methods in gut microbial community profiling.
    Article Snippet: .. A second amplification was performed using the Fluidigm AccessArray primers containing sample-specific barcodes and Illumina sequencing adapters. .. The second PCR was carried out in a total volume of 20 μl, containing 1 μl of DNA (taken directly from the product of the first PCR), 10 μl of 2× MyTaq HS Red Mix or AccuStart II PCR ToughMix, 4 μl of 0.4 μM Fluidigm AccessArray Barcoded primers for Illumina, and 5 μl of moleculargrade H2O.

    Article Title: Diazotroph Community Characterization via a High-Throughput nifH Amplicon Sequencing and Analysis Pipeline
    Article Snippet: .. In the final stage of PCR, amplicons from the second stage of PCR amplification were amplified using AccessArray primers (Fluidigm AccessArray barcode library; Fluidigm, San Francisco, CA, USA) to incorporate sample-specific barcodes and Illumina sequencing adapters. ..

    Article Title: Assessing the comparability of different DNA extraction and amplification methods in gut microbial community profiling
    Article Snippet: .. A second amplification was performed using the Fluidigm AccessArray primers containing sample-specific barcodes and Illumina sequencing adapters. .. The second PCR was carried out in a total volume of 20 μl, containing 1 μl of DNA (taken directly from the product of the first PCR), 10 μl of 2× MyTaq HS Red Mix or AccuStart II PCR ToughMix, 4 μl of 0.4 μM Fluidigm AccessArray Barcoded primers for Illumina, and 5 μl of molecular-grade H 2 O.

    Article Title: The microbial reproductive ecology of white-faced capuchins (Cebus capucinus).
    Article Snippet: Funding information Beckman Institute for Advanced Science and Technology, University of Illinois, UrbanaChampaign; Graduate Research Fellowship Program, National Science Foundation; Department of Anthropology, University of Illinois at Urbana-Champaign; Northwestern University; Center for Latin American Studies, University of Illinois at Urbana-Champaign; Lewis and Clark Fund for Exploration and Research, American Philosophical Society Changes in reproductive status influence energy and nutrient requirements in female primates.. The gut microbiota may buffer changes in energy demands, with shifts in community composition increasing the energy production potential of the gut during pregnancy and lactation.. In this study, we examine changes in the gut microbiome of wild, female white-faced capuchins (Cebus capucinus) across different reproductive states.

    Polymerase Chain Reaction:

    Article Title: Diazotroph Community Characterization via a High-Throughput nifH Amplicon Sequencing and Analysis Pipeline
    Article Snippet: .. In the final stage of PCR, amplicons from the second stage of PCR amplification were amplified using AccessArray primers (Fluidigm AccessArray barcode library; Fluidigm, San Francisco, CA, USA) to incorporate sample-specific barcodes and Illumina sequencing adapters. ..



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    Image Search Results


    Expected TP count at different multiplexing rates for SNVQ, Strelka, 2CP, and PLATO run using AutoML, spies-based classification threshold selection, and 50% bootstrap support. The dots represent TP counts from the actual AccessArray resequencing experiment reported in Table . P1–P4 denote the sequencing datasets generated for four different ovarian cancer patients

    Journal: BMC Bioinformatics

    Article Title: Semi-supervised learning for somatic variant calling and peptide identification in personalized cancer immunotherapy

    doi: 10.1186/s12859-020-03813-x

    Figure Lengend Snippet: Expected TP count at different multiplexing rates for SNVQ, Strelka, 2CP, and PLATO run using AutoML, spies-based classification threshold selection, and 50% bootstrap support. The dots represent TP counts from the actual AccessArray resequencing experiment reported in Table . P1–P4 denote the sequencing datasets generated for four different ovarian cancer patients

    Article Snippet: In all cases, the resequenced set included all variants that passed the 2CP filter and for which AccessArray primers could be successfully designed using the primer design tool in GeNeo.

    Techniques: Multiplexing, Selection, Sequencing, Generated